Selected Publications

A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors. Bohaciakova D, Hruska-Plochan M, Tsunemoto R, Gifford WD, Driscoll SP, Glenn TD, Wu S, Marsala S, Navarro M, Tadokoro T, Juhas S, Juhasova J, Platoshyn O, Piper D, Sheckler V, Ditsworth D, Pfaff SL, Marsala M. Stem Cell Res Ther. 2019 Mar 12;10(1):83. doi: 10.1186/s13287-019-1163-7.; PMID: 30867054
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A First-in-Human, Phase I Study of Neural Stem Cell Transplantation for Chronic Spinal Cord Injury. Curtis E, Martin JR, Gabel B, Sidhu N, Rzesiewicz TK, Mandeville R, Van Gorp S, Leerink M, Tadokoro T, Marsala S, Jamieson C, Marsala M, Ciacci JD. Cell Stem Cell. 2018 Jun 1;22(6):941-950.e6. doi: 10.1016/j.stem.2018.05.014.; PMID: 29859175
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Survival of syngeneic and allogeneic iPSC-derived neural precursors after spinal grafting in minipigs. Strnadel J, Carromeu C, Bardy C, Navarro M, Platoshyn O, Glud AN, Marsala S, Kafka J, Miyanohara A, Kato T Jr, Tadokoro T, Hefferan MP, Kamizato K, Yoshizumi T, Juhas S, Juhasova J, Ho CS, Kheradmand T, Chen P, Bohaciakova D, Hruska-Plochan M, Todd AJ, Driscoll SP, Glenn TD, Pfaff SL, Klima J, Ciacci J, Curtis E, Gage FH, Bui J, Yamada K, Muotri AR, Marsala M. Sci Transl Med. 2018 May 9;10(440). pii: eaam6651. doi: 10.1126/scitranslmed.aam6651.; PMID: 29743351
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Time-dependent, bidirectional, anti- and pro-spinal hyper-reflexia and muscle spasticity effect after chronic spinal glycine transporter 2 (GlyT2) oligonucleotide-induced downregulation. Kamizato K, Marsala S, Navarro M, Kakinohana M, Platoshyn O, Yoshizumi T, Lukacova N, Wancewicz E, Powers B, Mazur C, Marsala M. Exp Neurol. 2018 Jul;305:66-75. doi: 10.1016/j.expneurol.2018.03.013. Epub 2018 Mar 30.; PMID: 29608917
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Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice. Tadokoro T, Miyanohara A, Navarro M, Kamizato K, Juhas S, Juhasova J, Marsala S, Platoshyn O, Curtis E, Gabel B, Ciacci J, Lukacova N, Bimbova K, Marsala M. J Vis Exp. 2017 Jul 13;(125). doi: 10.3791/55770.; PMID: 28745630
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Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs. Miyanohara A, Kamizato K, Juhas S, Juhasova J, Navarro M, Marsala S, Lukacova N, Hruska-Plochan M, Curtis E, Gabel B, Ciacci J, Ahrens ET, Kaspar BK, Cleveland D, Marsala M.  Mol Ther Methods Clin Dev. 2016 Jul 13;3:16046. doi: 10.1038/mtm.2016.46. eCollection 2016.; PMID: 27462649
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Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization. Corleto JA, Bravo-Hernández M, Kamizato K, Kakinohana O, Santucci C, Navarro MR, Platoshyn O, Cizkova D, Lukacova N, Taylor J, Marsala M.  PLoS One. 2015 Dec 29;10(12):e0144642. doi: 10.1371/journal.pone.0144642. eCollection 2015.; PMID: 26713446
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Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies. Juhasova J, Juhas S, Hruska-Plochan M, Dolezalova D, Holubova M, Strnadel J, Marsala S, Motlik J, Marsala M.  Cell Mol Neurobiol. 2015 Jan;35(1):57-70. doi: 10.1007/s10571-014-0145-7. Epub 2014 Dec 9.; PMID: 25487013
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Translation of the rat thoracic contusion model; part 1-supraspinally versus spinally mediated pain-like responses and spasticity. van Gorp S, Deumens R, Leerink M, Nguyen S, Joosten EA, Marsala M.  Spinal Cord. 2014 Jul;52(7):524-8. doi: 10.1038/sc.2014.72. Epub 2014 May 13.; PMID: 24819511
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Translation of the rat thoracic contusion model; part 2 – forward versus backward locomotion testing. van Gorp S, Leerink M, Nguyen S, Platoshyn O, Marsala M, Joosten EA.  Spinal Cord. 2014 Jul;52(7):529-35. doi: 10.1038/sc.2014.73. Epub 2014 May 13.; PMID: 24819507
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Pig models of neurodegenerative disorders: Utilization in cell replacement-based preclinical safety and efficacy studies. Dolezalova D, Hruska-Plochan M, Bjarkam CR, Sørensen JC, Cunningham M, Weingarten D, Ciacci JD, Juhas S, Juhasova J, Motlik J, Hefferan MP, Hazel T, Johe K, Carromeu C, Muotri A, Bui J, Strnadel J, Marsala M.  J Comp Neurol. 2014 Aug 15;522(12):2784-801. doi: 10.1002/cne.23575. Epub 2014 Apr 12. Review.; PMID: 24610493
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Effective long-term immunosuppression in rats by subcutaneously implanted sustained-release tacrolimus pellet: effect on spinally grafted human neural precursor survival. Sevc J, Goldberg D, van Gorp S, Leerink M, Juhas S, Juhasova J, Marsala S, Hruska-Plochan M, Hefferan MP, Motlik J, Rypacek F, Machova L, Kakinohana O, Santucci C, Johe K, Lukacova N, Yamada K, Bui JD, Marsala MExp Neurol. 2013 Oct;248:85-99. doi: 10.1016/j.expneurol.2013.05.017. Epub 2013 Jun 5.; PMID: 23748136
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Amelioration of motor/sensory dysfunction and spasticity in a rat model of acute lumbar spinal cord injury by human neural stem cell transplantation. van Gorp S, Leerink M, Kakinohana O, Platoshyn O, Santucci C, Galik J, Joosten EA, Hruska-Plochan M, Goldberg D, Marsala S, Johe K, Ciacci JD, Marsala M.  Stem Cell Res Ther. 2013 May 28;4(3):57. doi: 10.1186/scrt209.; PMID: 23710605
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ALS-linked TDP-43 mutations produce aberrant RNA splicing and adult-onset motor neuron disease without aggregation or loss of nuclear TDP-43. Arnold ES, Ling SC, Huelga SC, Lagier-Tourenne C, Polymenidou M, Ditsworth D, Kordasiewicz HB, McAlonis-Downes M, Platoshyn O, Parone PA, Da Cruz S, Clutario KM, Swing D, Tessarollo L, Marsala M, Shaw CE, Yeo GW, Cleveland DW.  Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E736-45. doi: 10.1073/pnas.1222809110. Epub 2013 Feb 4.; PMID: 23382207
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Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation. Hefferan MP, Galik J, Kakinohana O, Sekerkova G, Santucci C, Marsala S, Navarro R, Hruska-Plochan M, Johe K, Feldman E, Cleveland DW, Marsala M.  PLoS One. 2012;7(8):e42614. doi: 10.1371/journal.pone.0042614. Epub 2012 Aug 20.; PMID: 22916141
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Development of AMPA receptor and GABA B receptor-sensitive spinal hyper-reflexia after spinal air embolism in rat: a systematic neurological, electrophysiological and qualitative histopathological study. Kakinohana O, Scadeng M, Corleto JA, Sevc J, Lukacova N, Marsala M.  Exp Neurol. 2012 Sep;237(1):26-35. doi: 10.1016/j.expneurol.2012.06.004. Epub 2012 Jun 18.; PMID: 22721766  View in  PubMed

Combinational spinal GAD65 gene delivery and systemic GABA-mimetic treatment for modulation of spasticity. Kakinohana O, Hefferan MP, Miyanohara A, Nejime T, Marsala S, Juhas S, Juhasova J, Motlik J, Kucharova K, Strnadel J, Platoshyn O, Lazar P, Galik J, Vinay L, Marsala M.  PLoS One. 2012;7(1):e30561. doi: 10.1371/journal.pone.0030561. Epub 2012 Jan 23.; PMID: 22291989
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Chronic spinal compression model in minipigs: a systematic behavioral, qualitative, and quantitative neuropathological study. Navarro R, Juhas S, Keshavarzi S, Juhasova J, Motlik J, Johe K, Marsala S, Scadeng M, Lazar P, Tomori Z, Schulteis G, Beattie M, Ciacci JD, Marsala M.  J Neurotrauma. 2012 Feb 10;29(3):499-513. doi: 10.1089/neu.2011.2076. Epub 2012 Jan 13.; PMID: 22029501  View in PubMed

Potent suppression of stretch reflex activity after systemic or spinal delivery of tizanidine in rats with spinal ischemia-induced chronic spastic paraplegia. Fuchigami T, Kakinohana O, Hefferan MP, Lukacova N, Marsala S, Platoshyn O, Sugahara K, Yaksh TL, Marsala M.  Neuroscience. 2011 Oct 27;194:160-9. doi: 10.1016/j.neuroscience.2011.08.022. Epub 2011 Aug 16.; PMID: 21871540;  View in PubMed

Optimization of immunosuppressive therapy for spinal grafting of human spinal stem cells in a rat model of ALS. Hefferan MP, Johe K, Hazel T, Feldman EL, Lunn JS, Marsala M.  Cell Transplant. 2011;20(8):1153-61. doi: 10.3727/096368910X564553. Epub 2011 Jun 9.; PMID: 21669047  View in PubMed

Analysis of dosing regimen and reproducibility of intraspinal grafting of human spinal stem cells in immunosuppressed minipigs. Usvald D, Vodicka P, Hlucilova J, Prochazka R, Motlik J, Kuchorova K, Johe K, Marsala S, Scadeng M, Kakinohana O, Navarro R, Santa M, Hefferan MP, Yaksh TL, Marsala M.  Cell Transplant. 2010;19(9):1103-22. doi: 10.3727/096368910X503406. Epub 2010 Apr 21.; PMID: 20412634  View in PubMed

Functional recovery in rats with ischemic paraplegia after spinal grafting of human spinal stem cells. Cizkova D, Kakinohana O, Kucharova K, Marsala S, Johe K, Hazel T, Hefferan MP, Marsala M.  Neuroscience. 2007 Jun 29;147(2):546-60. Epub 2007 May 23.; PMID: 17524565  View in PubMed

Measurement of peripheral muscle resistance in rats with chronic ischemia-induced paraplegia or morphine-induced rigidity using a semi-automated computer-controlled muscle resistance meter. Marsala M, Hefferan MP, Kakinohana O, Nakamura S, Marsala J, Tomori Z.  J Neurotrauma. 2005 Nov;22(11):1348-61.; PMID:16305323  View in PubMed

Spinal implantation of hNT neurons and neuronal precursors: graft survival and functional effects in rats with ischemic spastic paraplegia. Marsala M, Kakinohana O, Yaksh TL, Tomori Z, Marsala S, Cizkova D.  Eur J Neurosci. 2004 Nov;20(9):2401-14.; PMID: 15525281  View in PubMed

Loop dialysis catheter: a technology for chronic spinal dialysis in a freely moving rat. Marsala M.  Methods Mol Med. 2004;99:99-108.; PMID: 15131332  View in PubMed

Effect of proximal arterial perfusion pressure on function, spinal cord blood flow, and histopathologic changes after increasing intervals of aortic occlusion in the rat. Taira Y, Marsala M.  Stroke. 1996 Oct;27(10):1850-8.; PMID: 8841344  View in PubMed